Imaging techniques are crucial for observing and monitoring physiological or environmental changes at organ, tissue, cell, and molecular level. Non-invasive imaging modalities are particularly important for longitudinal animal studies.
The In Vivo Imaging facility of the Immunotherapy Development Center (IDC) provides imaging services and consultation on the planning, execution and analysis of fluorescence and bioluminescence experiments for TRON scientists and interested external groups.
Non-invasive evaluation of protein/molecule signals and luciferase gene expression in live laboratory animals is possible using in vivo fluorescence and bioluminescence imaging (FLI & BLI), fluorescence, respectively.
- Monitoring tumor growth
- Studying cell trafficking
- Tumor/tissue/cell targeting via antibodies
- Infectious diseases
- Characterization of transgenic animals
- Investigating transcriptional/translational regulation
In FLI and BLI, emitted light in the form of fluorescence or luminescence is detected by a system equipped with a charge-coupled device (CCD). The signal is processed and presented by a dedicated software application. Our core facility also provides consultation on the design and performance of FLI and BLI experiments and the analysis of the results.
At TRON we conduct:
- In vivo monitoring of tumor growth using various firefly luciferase expressing cell lines administered via different routes (e.g. i.v., s.c., orthotopic) subsequent to therapeutic anti-tumoral vaccination
- In vivo kinetics of firefly luciferase encoding in vitro transcribed RNA administered via different routes
- In vivo biodistribution of fluorescently labeled nanoparticles and liposomal formulations
- In vivo monitoring of transgene expression via BLI or FLI in transgenic animals