Multi-omics characterization of the 4T1 murine mammary gland tumor model

The murine mammary carcinoma cell line 4T1 is a widely used syngeneic tumor model for human breast cancer and specifically triple-negative breast cancer (TNBC). Profiling tumor models is important to translate pre-clinical findings appropriately. The authors aimed to complement the evaluation of 4T1 as adequate breast cancer or even TNBC model.

Analysis of the whole-exome sequencing data confirmed the similarity to human breast cancer with respect to mutation rate and mutational signatures. Also on the transcriptional level, 4T1 resembled human metastatic TNBC and in particular the basal-like and immune suppressed TNBC subtype. Out of 49 selected variants, 22 SNVs and one deletion identified in 4T1 triggered immune responses in immunized mice.

The presented genome, transcriptome and immunome data can thus serve as a baseline for further studies and supports the rational design of pre-clinical studies with an important and established tumor model. The generated NGS data is publicly available at the European Nucleotide Archive (PRJEB36287).

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