Fluorescence is the ability of a substance to absorb light of a given wavelength, and to emit light of another wavelength. In life sciences, fluorescence is used as a non-destructive way to track and analyze biomolecules and cells.
Applications that use fluorescence include:
– Flow cytometry
– DNA detection
A technique for counting and examining cells that have been labeled with antibodies. The labeled cells are suspended in a fluid stream (flow) and pass by a laser beam. The laser induces fluorescent signals on every cell that has bound to the antibody.
fluorescence activated cell sorting
A special type of flow cytometry method, in which a single cell or a mixture of cells are sorted based on their characteristics.