Glossary – E

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E

electroporation
Electroporation is a widely used gene transfer method. Cells are placed for a very short time in a strong electric field, which induces pores in the cell wall. DNA and other molecules enter the cells via these pores.

ELISA
See enzyme-linked immunosorbent assay

enzyme
Enzymes are proteins that act as biological catalysts. They facilitate biochemical reactions and are localized both inside cells and outside cells in body fluids.

ELISPOT
See enzyme-linked immunosorbent spot

enzyme-linked immunosorbent assay
A technique that for detecting antibodies or antigens in a sample. The method uses both antibodies and enzymes for detection. The enzyme catalysis changes the color of a substrate and thereby allows the visualization and quantification of the sample.

enzyme-linked immunosorbent spot
This technique is very similar to the ELISA. It is used to visualize proteins that are secreted by cells. The proteins are captured right after secretion and are visualized after the cell has been removed. A spot appears where the cell was previously located.