Mainz, Germany, June 10, 2014 – In the second edition of the highly acclaimed reference book “Handbook of RNA Biochemistry”,TRON researcher Dr. John Castle addresses methods of transcriptome sequencing by next-generation sequencing. He is one of 100 specialists from all over the world who share their up-to-date expertise and experiences, including hundreds of protocols, complete with explanations, and hitherto unpublished troubleshooting hints.
The book covers all modern techniques for the handling, analysis and modification of RNAs and their complexes with proteins. Throughout, the articles keep the practicing bench scientist in mind, providing quick and reliable access to a plethora of solutions for practical questions of RNA research, ranging from simple to highly complex.
Next generation sequencing of the transcriptome (NGS RNA-Seq) generates billions of short sequence reads. Castle presents computational workflows to make sense of these terabytes of data and accurately determine gene expression values. The chapter addresses read alignments, association with transcripts, uncertainty, normalization and data viewing.
Computational Methods for Gene Expression Profiling Using Next-Generation Sequencing (RNA-Seq)
Castle, J. C. (2014) Computational Methods for Gene Expression Profiling Using Next-Generation Sequencing (RNA-Seq), in Handbook of RNA Biochemistry: Second, Completely Revised and Enlarged Edition (eds R. K. Hartmann, A. Bindereif, A. Schön and E. Westhof), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany. doi: 10.1002/9783527647064.ch36